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电镜的高分辨和高放大性能,能够精确反映被观察客体真实结构。传统电子显微术强调,活体标本由离开母组织至固定的间隔时间应在几十秒之内,一般不能超过1分钟。这种取材原则,是试图避免死后自溶造成人工假象,从而达到保持组织细胞各种成份的天然结构的目的。但在生理学上,任何生活细胞对取材的机械等一些刺激都会发生不同程度的应激反应。这在具有收缩功能的心肌、骨骼肌和平滑肌等组织表现得最为显著,以致常常难以与病理性反应相鉴别,甚至造成诊断错误。本文就
High-resolution electron microscopy and high magnification properties, can accurately reflect the true structure of the observed object. Traditional electromicroscopy emphasizes that living specimens should leave the mother’s tissue at fixed intervals within tens of seconds and generally can not exceed 1 minute. This principle is based on the principle of avoiding artificial autopsy caused by post mortem, so as to achieve the purpose of maintaining the natural structure of various components of tissue cells. However, in physiology, any living cells may experience different degrees of stress response to some stimuli such as mechanical drawing. This systolic function in the myocardium, skeletal muscle and smooth muscle and other organizations showed the most significant, so often difficult to distinguish and pathological reactions, and even cause diagnostic errors. This article is