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目的观察红珊瑚对实验大鼠骨折愈合过程的影响。方法 60只SPF级Wistar大鼠随机取12只为空白对照组,其余大鼠造成左前肢桡骨骨折模型,经X射线影像透视后确定大鼠桡骨骨折模型建立成功。将骨折模型大鼠随机分为模型组、阳性药物(伤科接骨片,150μg/g)组、红珊瑚高剂量组(300μg/g)、红珊瑚低剂量组(150μg/g),每组12只。造模后第2 d开始灌胃药,每天1次。给药后第10 d、20 d、30 d分别对每组大鼠骨折部位进行X射线影像评估,骨折部位HE染色后观察组织病理改变,并用酶联免疫法(ELISA)测定血清中碱性磷酸酶(ALP)活性及钙(Ca)、磷(P)含量。结果 X射线影像显示,给药第10 d时红珊瑚高剂量组大鼠骨折端边缘线变模糊;20 d时骨折断端骨痂增多,密度增加,边缘接近消失;30 d时骨痂填满骨折缺损,完全愈合。骨折部位HE染色可以看出红珊瑚高剂量组大鼠骨折处纤维组织多,骨痂形成速度较其他组更快,红珊瑚高剂量组的骨折愈合周期较其他组更短。红珊瑚高剂量组血清中Ca、P水平逐渐升高,并明显高于模型组,差异均有统计学意义(P<0.05)。ALP含量有先升后降趋势,较其他组先出现且明显,差异有统计学意义(P<0.05)。结论红珊瑚能够调节大鼠血清ALP和Ca、P水平,加速钙盐沉积,促进大鼠骨折部位纤维组织增多,加速骨痂形成,缩短骨折愈合周期,对骨折的愈合和修复有促进作用。
Objective To observe the effect of red coral on fracture healing in experimental rats. Methods Totally 60 SPF Wistar rats were randomly divided into blank control group and the other rats were subjected to radial fracture of the left forelimb. The model of radius fracture was established after X-ray fluoroscopy. The fracture model rats were randomly divided into model group, positive drug (Shangkejiegu tablets, 150μg / g), red coral high dose group (300μg / g), red coral low dose group (150μg / g) only. 2d after the beginning of intragastric administration of drugs, 1 day. X-ray images of the fracture sites of each group were evaluated on the 10th, 20th, and 30th day after the administration. Histopathological changes were observed after the fracture site was stained with HE. Serum alkaline phosphatase (ALP) activity and calcium (Ca), phosphorus (P) content. Results The X-ray images showed that on the 10th day after administration, the red edge of the high-dose coral redness group became obscured, and at 20 days, the callus increased, the density increased and the edge disappeared. At 30 days, the callus was filled Fracture defect, completely healed. Hematoxylin-eosin staining showed that there were many fibrous tissue fractures in the high-dose Honglvhu high-dose group, and the formation rate of callus was faster than that in other groups. The fracture healing period of high-dose Honglvhu group was shorter than that of other groups. The levels of Ca and P in the high-dose red coral group increased gradually and were significantly higher than those in the model group (P <0.05). ALP content increased first and then decreased, compared with the other groups appeared first and obvious, the difference was statistically significant (P <0.05). CONCLUSION Red coral can regulate ALP and Ca, P levels in serum, accelerate deposition of calcium salts, increase fibrous tissue at the fracture site, accelerate the formation of callus, shorten the healing period of fractures, and promote the healing and repair of fractures.