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胃粘膜异型增生(dysplasia)已公认是一种癌前病变,具有癌变的潜在趋向,本文应用胃癌单克隆抗体MG7和菜豆凝集素PHA_L对胃粘膜异型增生和胃癌进行对比研究,探讨两者的关系。1 材料和方法 本文选用1986～1988年间胃镜检查粘膜活检诊断的正常胃粘膜20例,胃粘膜异型增生100例,(按Ming和Morson等标准将异型增生分为轻度20例,中度60例,重度20例);胃癌30例;所有组织均用常规福尔马林固定,石蜡包埋。试剂:①胃癌单克隆抗体MG7由第四军医大学馈赠。②菜豆凝集素PHA_L及ABC试剂盒为美国Vector公司产品。染色方法用ABC法进行。
Gastric mucosa dysplasia (dysplasia) has been recognized as a precancerous lesion and has a potential trend of canceration. In this study, gastric cancer dysplasia and gastric cancer were compared using monoclonal antibody MG7 and analectin PHA_L, and the relationship between the two was discussed. . 1 Materials and Methods This article selected 20 cases of normal gastric mucosa diagnosed by gastroscopy biopsy from 1986 to 1988, and 100 cases of dysplasia of gastric mucosa. According to the criteria of Ming and Morson, dysplasia was divided into mild 20 cases and moderate 60 cases. , Severe in 20 cases); 30 cases of gastric cancer; all tissues were fixed in regular formalin and embedded in paraffin. Reagents: 1 Gastric cancer monoclonal antibody MG7 is a gift from the Fourth Military Medical University. 2 The bean lectin PHA_L and ABC kits were products of the Vector Company of the United States. The staining method was performed using the ABC method.