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以微藻类小新月菱形藻(Nitzschia closterium)为研究对象,通过设置不同的原油分散液(WAF)浓度,分别进行了急性(4 d)和亚急性(29 d)毒理实验;通过细胞计数、丙二醛(MDA)测定、稳定同位素组成分析等方法测定了原油分散液(WAF)对小新月菱形藻生长曲线、C和N稳定同位素组成(δ13C、δ15N值)的影响。结果表明,WAF在低浓度(≤1.0 mg/L)长期作用下对小新月菱形藻的生长有促进作用,而较高浓度(≥5.0 mg/L)则使藻细胞生长受到抑制;小新月菱形藻的丙二醛含量随时间及WAF浓度增大而逐渐增加,最高可达1.65倍。WAF在低浓度(≤1.0 mg/L)作用下,藻细胞的δ13C、δ15N值都趋于增大,而较高浓度(≥5.0 mg/L)则使藻细胞的δ13C、δ15N值趋于变小,这表明,微藻类的C、N稳定同位素组成能够从一定程度上反映污染程度,因此可作为海洋溢油污染监测的一种新指标。
Acute (4 d) and subacute (29 d) toxicological experiments were performed on different microalgae Nitzschia closterium strains by setting different concentrations of crude oil dispersions (WAF) , Malondialdehyde (MDA) and stable isotope composition analysis were used to determine the effects of crude oil dispersion (WAF) on the growth curve, C and N stable isotopic composition (δ13C, δ15N) of A. crenata. The results showed that WAF could promote the growth of A. crenulatum at low concentration (≤1.0 mg / L), but inhibited the growth of A. alga at a higher concentration (≥5.0 mg / L) The content of malondialdehyde in M. lilium increased with the increase of time and WAF concentration up to 1.65 times. The δ13C and δ15N values of algae cells tended to increase with WAF at the concentration of 1.0 mg / L, while the δ13C and δ15N values of algae cells tended to change with higher concentration (≥5.0 mg / L) This indicates that the C, N stable isotopic composition of microalgae can reflect the degree of pollution to a certain extent, so it can be used as a new indicator for marine oil spill pollution monitoring.