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为了解医院高压灭菌器的消毒效果,加强医院消毒工作,于1994年4～8月,对青岛市30家医院的卧式下排气高压灭菌器进行检测,结果报告如下:1 材料与方法1.1 实验材料及判断标准 嗜热脂肪杆菌芽胞菌片,由北京军事医学科学院四环制药厂生产,每片染菌量为5X10~5三M胶带,美国产,上海总经销.灭菌后的菌片按《消毒技术规程》要求进行检验,阴性者为合格;灭菌后三M胶带达到变色标准为合格.1.2 测试方法按《消毒技术规范》要求制作标准试验包.测试时在每个包的中心放置两片嗜热脂肪杆菌芽胞菌片,三M胶带贴在标准包的外边.采用五点放置法,于每具灭菌器的上层和中层各放一个包,下层的前、中、后各放一个包.加热必须达到121℃,压力不超过137.30kPa,维持30分钟.
In order to understand the disinfection effect of the hospital autoclave and strengthen the hospital’s disinfection work, from April to August 1994, the horizontal lower exhaust autoclave at 30 hospitals in Qingdao was tested. The results are reported as follows: 1 Materials and Method 1.1 Experimental Materials and Criterion The standard Bacillus stearothermophilus spores were produced by the Fourth Ring Pharmaceutical Factory of the Beijing Academy of Military Medical Sciences. The amount of bacteria in each piece was 5X10~5 3M tape, which was produced in the United States and sold in Shanghai. After sterilization. The bacteria tablets are inspected according to the requirements of the “Disinfection Technical Regulations”, and the negative ones are qualified; after the sterilization, the three M tapes meet the color-changing standards as qualified. 1.2 The test methods are made according to the “Disinfection Technical Specifications” standard test kits. Each packet is tested during the test. Place two pieces of Bacillus stearothermophilus spores in the center, three M tapes are placed on the outside of the standard bag. Use a five-point placement method, put a bag in the upper and middle layers of each sterilizer, the lower, front, middle, After each put a package. Heating must reach 121 °C, the pressure does not exceed 137.30kPa, maintain 30 minutes.