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目的:了解粘放菌两种菌毛在粘附牙面和与血链球菌34凝集中的生物活性。方法:采用粘放菌Ⅰ型和Ⅱ型菌毛提取物及两种抗菌毛抗体,通过它们对粘放菌粘附唾液包被的羟磷灰石(SHA)的抑制实验来判断两种菌毛的粘附活性,通过粘放菌与血链球菌34的凝集实验及两种抗菌毛抗体对凝集反应的抑制实验来确定两种菌毛的凝集活性。结果:①粘放菌Ⅰ型和Ⅱ型菌毛提取物及两种抗菌毛抗体均能抑制粘放菌对SHA的粘附;②只有Ⅱ型菌毛能间接引起肉眼可见的血链球菌34的凝集反应,抗Ⅱ型菌毛抗体能抑制凝集反应,抗Ⅰ型菌毛抗体无此抑制作用。结论:Ⅰ型和Ⅱ型菌毛均有粘附性能;Ⅱ型菌毛有凝集性能,Ⅰ型菌毛无凝集性能;本课题所采用的菌毛分离法未破坏菌毛的生物活性。
OBJECTIVE: To understand the biological activity of two kinds of pilus pili in agglutinating tooth surface and agglutinating with Streptococcus sanguis 34. Methods: Two types of pili were determined by the inhibition experiments on the adhesion of salivary mucoadhesive hydroxyapatite (SHA) The agglutination activity of the two pili was determined by the agglutination test of the adhesion bacteria and Streptococcus sanguis 34 and the inhibition test of the agglutination reaction of the two antifungal antibodies. Results: ①Sophilus type Ⅰ and type Ⅱ pili extracts and two kinds of anti-pilus antibodies could inhibit the sticky bacteria adhesion to SHA; ② Only type Ⅱ pilus can indirectly cause macroscopic streptococcus sanguis 34 Agglutination reaction, anti-type pili antibody can inhibit agglutination, anti-type pili antibody does not inhibit. Conclusion: Type Ⅰ and type Ⅱ pili have adhesive properties. Type Ⅱ pili has the ability of agglutination and type Ⅰ pili without agglutination. The pili separation method used in this project does not destroy the biological activity of pili.