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目的利用超高效液相色谱技术,建立保健食品中木香烃内酯和去氢木香烃内酯含量的检测方法。方法样品经甲醇提取,采用ACQUITY UPLC BEH C18色谱柱(2.1 mm×50 mm,1.7μm),以乙腈-水(40∶60,V/V)为流动相,紫外检测器波长为225 nm,流速为0.5 ml/min,进样体积为5μl,柱温为30℃,通过超高效液相色谱分离,以外标法定量。结果木香烃内酯和去氢木香烃内酯的浓度为0μg/ml~1 000μg/ml时,标准曲线的线性关系良好,线性相关系数为0.999 9。相对标准偏差(RSD)为0.5%~3.7%,加标回收率分别为96.2%~100.6%、96.2%~103.9%。结论本方法具有灵敏度高、选择性好、精密度和准确度好等优点,而且更快捷,适合保健食品的日常检测工作。
OBJECTIVE To establish a method for the determination of the content of woodlocolactone and dehydrocostuslactone in health food by using ultra performance liquid chromatography (HPLC). Methods The samples were extracted with methanol and analyzed by ACQUITY UPLC BEH C18 column (2.1 mm × 50 mm, 1.7 μm) using acetonitrile-water (40:60, V / V) as mobile phase. The UV detector wavelength was 225 nm. 0.5 ml / min, injection volume 5μl, column temperature 30 ℃, separated by ultra performance liquid chromatography and quantified by external standard method. Results The linearity of the standard curve was good with the linear range of 0.999 9 for the linear range of the calibration curve for the range of 0 to 1,000 μg / ml for both woody lactone and dehydrocostus lactone. The relative standard deviations (RSDs) ranged from 0.5% to 3.7%. The spiked recoveries were 96.2% ~ 100.6% and 96.2% ~ 103.9%, respectively. Conclusion This method has the advantages of high sensitivity, good selectivity, good precision and accuracy, and more efficient, suitable for routine testing of health food.