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探讨 9 1C3分子对杀伤细胞功能的影响。方法 :以新鲜分离的PBMC或混合淋巴细胞培养活化的淋巴细胞为效应细胞 ,采用重导向杀伤实验 (Redirectedkillingassay ,RKA)观察 9 1C3抗体对效应细胞杀伤P815细胞作用及其杀伤过程中TNF 浕分泌的影响?峁?:9 1C3抗体能显著抑制杀伤细胞对靶细胞的细胞毒作用 ,并使效应细胞分泌TNF 浕水平降低。结论 :9 1C3分子是一种抑制型杀伤细胞受体。
To investigate the effect of 9 1C3 on the function of killer cells. Methods: Freshly isolated PBMCs or mixed lymphocytes were used to culture activated lymphocytes as effector cells. The effects of 9 1C3 antibody on P815 cells and the secretion of TNF-γ during killing were observed by Redirected killing assay (RKA) Effect? 峁?: 9 1C3 antibody can significantly inhibit the cytotoxic effect of killer cells on target cells, and the effector cells to secrete lower levels of TNF 浕. Conclusion: 9 1C3 is an inhibitory killer cell receptor.