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【目的】2009年春季,浙江省舟山地区养殖鮸鱼暴发弧菌病。症状主要表现为体表病灶部位出血、肌肉溃烂、内脏器官有白斑等。【方法】从病鱼体表溃疡部位及内脏分离出优势菌株090212,经人工感染证实该菌即为致病菌。通过API系统和菌体常规形态特征、培养特性和生理生化反应指标测定以及16S rRNA测序分析等综合鉴定,【结果】确认090212为哈维氏弧菌(Vibrio harveyi),该菌为革兰氏阴性,菌体呈短杆状,极生单鞭毛。该菌对氟苯尼考、四环素等5种抗生素敏感。【结论】哈维氏弧菌是海水养殖鱼类的常见致病菌,但作为养殖鮸鱼的病原菌尚属首次报道,将对鮸鱼的病害防治和健康养殖具有重要的指导意义。
【Purpose】 In the spring of 2009, vibriosis of catfish was raised in Zhoushan, Zhejiang Province. Symptoms mainly manifested as bleeding on the surface lesions, muscle fester, visceral organs, such as white spots. 【Method】 The dominant strain 090212 was isolated from ulcers and viscera of diseased fish, which was proved to be pathogenic by artificial infection. Through the API system and conventional morphological characteristics of bacteria, culture characteristics and physiological and biochemical reaction indicators and 16S rRNA sequencing analysis and other comprehensive identification, [Results] confirmed 090212 for Vibrio harveyi (Vibrio harveyi), the bacteria is Gram-negative , The cell was short rod-shaped, polar single flagella. The bacteria florfenicol, tetracycline and other five kinds of antibiotics sensitive. 【Conclusion】 Vibrio harveyi is a common pathogen of mariculture fish. However, it is the first reported pathogen of mandarin fish, which will be of great significance to the prevention and cure of mandarin fish diseases and healthy breeding.