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应用八倍体小麦-簇毛麦和普通小麦杂交、回交,试图将其抗白粉病基因导入普通小麦。通过细胞学分析和染色体分带分析证明双二倍体小簇麦含有56条染色体,其染色体组为AABBDDVV,其与普通小麦的杂种F_1代减数分裂的中期I二价体数目常少于21对,单价体数目常多于7个,并出现三价体、四价体和少量五价体。应用生物素标记的簇毛麦总DNA探针对小簇麦杂种进行染色体原位杂交,能够清楚检测出簇毛麦的染色体,双二倍体小簇麦含有14条簇毛麦染色体,94009—5—4含有V_2,V_3和V_43条簇毛麦染色体,94009-5-9含有1条簇毛麦的V_6染色体。目前已选育了优良抗病品系,经温室和田间人工接种鉴定,表现高抗小产白粉病。
Haploid Wheat-Hirsutum (Triticum aestivum L.) was crossed with common wheat (Triticum aestivum L.) and backcrossed to try to introduce the powdery mildew resistance gene into common wheat. Cytological analysis and chromosome segregation analysis showed that the diploid small cluster wheat contained 56 chromosomes and its chromosome group was AABBDDVV. The number of metaphase I digests of the meiotic F 1 generation of common wheat was less than 21 Yes, the number of monovalent often more than 7, and appeared trivalent, tetravalent and a small amount of pentavalent. Using biotinylated total RNA probe of cluster hamster to detect the chromosomes of small cluster wheat hybrids, the chromosomes of cluster wheat can be detected clearly. The diploid small cluster wheat contains 14 chromosomes, 5-4 contains V 2, V_3 and V_43 rammer chromosomes, and 94009-5-9 contains one V_6 chromosome. At present, we have selected the excellent resistant lines and identified them by artificial inoculation in greenhouse and field, showing high resistance to powdery mildew.