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目的:研究刺五加多糖(ASPS)对由过氧化氢(H2O2)氧化损伤的大鼠海马神经元内氧自由基的影响。方法:运用海马神经细胞原代培养技术,采用H2O2诱导建立细胞氧化损伤模型。观察细胞形态学变化;甲基四氮唑盐(MTT)法测定细胞活性;免疫组化法鉴定海马神经元,化学比色法测定丙二醛(MDA)、H2O2、羟自由基(ROS)含量及细胞外液中乳酸脱氢酶(LDH)的活性。结果:形态学观察显示,与模型组比较,ASPS组细胞损伤程度明显减轻;ASPS组细胞活性(0.46)比模型组细胞活性(0.36)高(P<0.01);MDA、H2O2、ROS的含量及细胞外液中LDH的活力ASPS组明显低于模型组。结论:ASPS能降低海马神经元中氧自由基的含量,并对海马神经元氧化损伤有保护作用。
Objective: To study the effects of Acanthopanax senticosus polysaccharide (ASPS) on oxygen free radicals in hippocampal neurons injured by hydrogen peroxide (H2O2). Methods: Primary culture of hippocampal neurons was used to establish a cell model of oxidative damage induced by H2O2. The morphological changes of cells were observed. The cell viability was measured by MTT assay. The hippocampal neurons were identified by immunohistochemistry. The contents of malondialdehyde (MDA), hydrogen peroxide (H2O2) and hydroxyl free radical (ROS) And extracellular lactate dehydrogenase (LDH) activity. RESULTS: Morphological observation showed that compared with the model group, the degree of cell injury in ASPS group was significantly reduced. The cell viability in ASPS group (0.46) was higher than that in model group (0.36) (P <0.01), and the content of MDA, H2O2 and ROS LDH activity in extracellular fluid ASPS group was significantly lower than the model group. CONCLUSION: ASPS can reduce the content of oxygen free radicals in hippocampal neurons and protect the hippocampal neurons from oxidative damage.