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为明确不同寄主植物对桃小食心虫(Carposina sasakii Matsumura)主要代谢物质的影响,选取6种寄主植物(大枣、山楂、梨、桃、苹果、杏)饲喂的桃小食心虫,分别使用过碘酸钠法、硫酸蒽酮比色法和盐酸水解法测定其体内甘油、海藻糖和氨基酸含量;通过定磷法和2,4-二硝基苯肼法测定Na+-K+-ATP酶和LDH酶的活性。结果表明:桃小食心虫体内主要代谢物质含量在不同寄主植物间存在差异,取食山楂的桃小食心虫甘油含量最高(12.48μg/mg),取食大枣次之(12.44μg/mg),取食杏的含量最低(10.43μg/mg);海藻糖含量为取食大枣的最高(27.16μg/mg),取食杏的最低(9.86μg/mg);取食6种寄主的桃小食心虫体内均检测到16种氨基酸,取食山楂的桃小食心虫体内天冬氨酸Asp含量高于取食其他5种寄主植物的;超微量Na+-K+-ATP酶活性测定结果显示取食桃的桃小食心虫活性最高(1.31 U/g pro),LDH酶活性以取食杏的最高(42.73 U/g pro)。
In order to clarify the effects of different host plants on the major metabolites of Carposina sasakii Matsumura, 6 peach moths fed on 6 host plants (jujube, hawthorn, peach, apple, apricot) were selected, The contents of glycerol, trehalose and amino acids in the body were determined by acid-sodium method, anthrone sulfuric acid colorimetry and hydrochloric acid hydrolysis method. The activities of Na + -K + -ATPase and LDH enzyme were determined by the method of P and 2,4-dinitrophenylhydrazine Activity. The results showed that the contents of major metabolites in host plants were different among different host plants. The content of glycerol was the highest (12.48μg / mg) and the second was 12.44μg / mg The content of trehalose was the lowest (10.46μg / mg), the highest content of trehalose (27.16μg / mg) was taken on jujube and the lowest was taken on apricot (9.86μg / mg) Sixteen kinds of amino acids were detected in vivo. The Asp content of aspartate in the peach moth fed on hawthorn was higher than that in the other five host plants. The results of ultra-trace Na + -K + -ATPase activity showed that peach peach peach The bollworm activity was the highest (1.31 U / g pro), and the LDH enzyme activity was highest (42.73 U / g pro).