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作者在用Abbott丙型肝炎病毒（HCV）酶免疫测定（EIA）2.0试剂盒检测pH4Gamimune N静脉注射用免疫球蛋白（IGIV）制品中的HCV抗体时发现,偶有假阳性结果,推测可能由于在低pH（pH4.0）时蛋白分子更易折叠,分子构象更紧密,导致分子的疏水性增加,对固相的亲和力增强。为了证实这一推测,作者采用尼罗红疏水性染料结合试验,即将随机抽取的5％和10％IGIV样本分成两份,1份用1N NaOH调pH至7.0,另1份维持pH4.0不变,然后均在5℃或37℃培育4天后,加入尼罗红疏水性染料测定蛋白分子的疏水性,试验同时排除了温度升高对HCV抗体稳定性的影响。
The authors found that occasional false-positive results in the detection of HCV antibodies in the IV product of IV immunization with GIVIMIM N N (IGIV) using the Abbott Hepatitis C Virus (HCV) Enzyme Immunoassay (EIA) 2.0 kit were presumably due to Protein molecules are more easily folded at lower pH (pH4.0) and have a more conformational conformation, leading to an increase in the hydrophobicity of the molecule and an increased affinity for the solid phase. To confirm this hypothesis, the authors used a Nile Red hydrophobic dye binding assay, which split the randomly sampled 5% and 10% IGIV samples into two aliquots, one adjusted to pH 7.0 with 1N NaOH and the other maintained at pH 4.0 After incubation at 5 ° C or 37 ° C for 4 days, the hydrophobicity of protein molecules was determined by adding Nile red hydrophobic dye. At the same time, the effect of elevated temperature on the stability of HCV antibody was excluded.