论文部分内容阅读
目的建立肾动脉狭窄性高血压食蟹猴模型,并制定其评价标准。方法选取7只正常成年雄性食蟹猴,使用丝线及针头狭窄肾动脉,运用两肾一夹(2K1C)法,制作肾动脉狭窄性高血压食蟹猴模型,并对模型进行评价,同时检测血生化相关指标。结果食蟹猴血压变化:术后1周血压开始升高,4周后达到稳定水平,平均血压为(18.70±1.30)/(10.62±0.91)kPa,食蟹猴术后血压显著高于术前(P<0.05),并且可维持至本实验持续时间8周。食蟹猴血生化变化:尿素、肌酐、谷丙转氨酶、谷草转氨酶术后7d明显升高,与术前比较差异有统计学意义(P<0.05);总胆固醇与葡萄糖升高不明显,与术前比较差异无统计学意义(P>0.05)。造模成功率约为71.43%。结论运用2K1C法成功建立肾动脉狭窄性高血压食蟹猴模型,该模型经济、操作简单易行、成功率高、稳定性好。
Objective To establish a cynomolgus monkey model of renal artery stenosis hypertension and to develop its evaluation criteria. Methods Seven normal adult male cynomolgus monkeys were selected, their renal arteries were stenosed by silk thread and needle, and 2K1C method was used to make cynomolgus monkey model of renal artery stenosis hypertension. The model was evaluated and the blood samples were tested for blood Biochemical related indicators. Results The blood pressure of cynomolgus monkeys increased at 1 week after operation and reached a stable level after 4 weeks. The average blood pressure was (18.70 ± 1.30) / (10.62 ± 0.91) kPa, and the blood pressure of cynomolgus monkey was significantly higher than that before operation (P <0.05), and can be maintained for the duration of this experiment for 8 weeks. Blood biochemical changes in cynomolgus monkeys: Urea, creatinine, alanine aminotransferase and aspartate aminotransferase increased significantly at 7th day after operation, showing significant difference from preoperative value (P <0.05); total cholesterol and glucose did not increase significantly, Before the difference was not statistically significant (P> 0.05). Modeling success rate of about 71.43%. Conclusion 2K1C method is successfully used to establish cynomolgus monkey model of renal artery stenosis hypertension. The model is simple in economy, simple in operation, high in success rate and good in stability.