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目的利用亲水作用液相色谱-电喷雾离子源质谱联用(HILIC/ESI-MS)对白花蛇舌草药材中的强极性组分进行考察。方法在电喷雾离子源负离子模式下,采用基于基峰强度的特征色谱图谱中的8个共有峰为考察对象,对不同产地的白花蛇舌草及其伪品水线草中的强极性组分进行主成分分析。结果 8个共有峰的准分子离子或加合分子离子的质荷比分别为:m/z 383.2,m/z 399.2,m/z 389.1,m/z 850.9,m/z 665.3,m/z 827.4,m/z 989.4,m/z 1151.6。通过主成分分析,白花蛇舌草药材中的强极性组分具有明显的地域性,且其与伪品水线草成分差异也十分显著。结论 HILIC/ESI-MS方法可以提供强极性组分一些基本信息,较单纯利用色谱保留时间为参数的指纹图谱分析更加可靠、准确,为进一步了解白花蛇舌草强极性组分提供依据。
OBJECTIVE To investigate the strong-polar components of Hedyotis diffusa Willd. By using hydrophilic interaction liquid chromatography-electrospray ionization mass spectrometry (HILIC / ESI-MS). Methods In the negative ion mode of electrospray ionization source, eight common peaks in the chromatogram based on base peak intensity were used as the investigation object. Sub-principal component analysis. Results The mass-to-charge ratios of quasi-molecular ions or adduct ion of eight common peaks were: m / z 383.2, m / z 399.2, m / z 389.1, m / z 850.9, m / z 665.3, m / z 827.4 , m / z 989.4, m / z 1151.6. Through the principal component analysis, the strong polar components in the Hedyotis diffusa will have obvious regional characteristics, and the difference between them is also significant. Conclusion The HILIC / ESI-MS method can provide some basic information of the strongly polar component, which is more reliable and accurate than the fingerprint analysis using only the chromatographic retention time as a parameter, and provides a basis for further understanding of the polar component of Hedyotis diffusa.