论文部分内容阅读
目的研究新疆维吾尔自治区维吾尔族胃癌患者血清胃蛋白酶原(pepsinogen,PG)与促胃液素-17(gastrin-17,G-17)的表达及其诊断价值,探讨维吾尔族胃癌患者在胃癌不同阶段及不同部位胃癌血清PG及G-17的表达特征及规律。方法将2014年1月至2015年10月于新疆维吾尔自治区喀什地区第二人民医院经内镜检查或手术及病理学确诊的维吾尔族胃癌患者198例(连续3代均为同一民族)纳入研究组,将同期58例维吾尔族健康体检者纳入对照组。采用酶联免疫吸附测定(ELISA)检测两组研究对象血清PGⅠ、PGⅡ及G-17水平,并计算PGⅠ与PGⅡ比值(PGR)。采用~(13)C或~(14)C-尿素呼气试验及粪便幽门螺杆菌(H.pylori)抗原检测法检测两组研究对象H.pylori感染情况。结果①研究组患者血清PGⅠ及PGR均明显低于对照组(P<0.01),而血清PGⅡ和G-17水平均明显高于对照组(P<0.01和P<0.05):②研究组患者H.pylori感染率明显高于对照组(P<0.05);③研究组中早期胃癌组患者血清PGⅠ水平及PGR均高于进展期胃癌组(P<0.01),而早期胃癌组患者G-17水平明显低于进展期胃癌组(P<0.05)。④研究组中胃窦癌组患者血清PGⅠ水平、PGR均高于胃体癌组和近端胃癌组(P<0.05),而胃体癌组与近端胃癌组患者血清G-17水平均高于胃窦癌组(P<0.05);胃体癌组与近端胃癌组患者之间血清PGⅠ、G-17水平及PGR比较均无明显差异(P>0.05)。结论血清PGⅠ、G-17水平及PGR可以作为新疆维吾尔自治区维吾尔族人群筛查和辅助诊断胃癌的血清学指标;血清PGⅠ、G-17水平及PGR变化与维吾尔族胃癌患者的肿瘤分期及不同病变部位具有相关性。
Objective To investigate the expression of pepsinogen (PG) and gastrin-17 (G-17) in Uygur patients with gastric cancer in Xinjiang Uygur Autonomous Region and its diagnostic value. The Characteristics and Regularities of Expression of Serum PG and G-17 in Different Parts of Gastric Cancer. Methods A total of 198 Uygur patients with gastric cancer diagnosed by endoscopy or surgery and pathology from the 2nd People’s Hospital of Kashi Prefecture in Xinjiang Uyghur Autonomous Region from January 2014 to October 2015 were enrolled in the study group In the same period, 58 cases of Uygur healthy subjects were included in the control group. Serum levels of PGⅠ, PGⅡ and G-17 were measured by enzyme-linked immunosorbent assay (ELISA), and the ratio of PGⅠ to PGⅡ (PGR) was calculated. The H.pylori infection in both groups was detected by ~ (13) C or ~ (14) C-urea breath test and H.pylori antigen test. Results ① The levels of serum PGⅠ and PGR in study group were significantly lower than those in control group (P <0.01), while the levels of PGⅡ and G-17 in serum were significantly higher in study group than those in control group (P <0.01 and P <0.05) (P <0.05); ③In the study group, the serum PGⅠ level and PGR in patients with early stage gastric cancer were significantly higher than those in advanced stage gastric cancer (P <0.01), while the level of G-17 in patients with early stage gastric cancer was significantly higher than that in control group Significantly lower than that of advanced gastric cancer group (P <0.05). (4) Serum levels of PGⅠ and PGR in patients with gastric cancer in study group were significantly higher than those in patients with gastric cancer and proximal gastric cancer (P <0.05), while serum levels of G-17 in patients with gastric cancer and proximal gastric cancer were significantly higher (P <0.05). There was no significant difference in serum PGⅠ, G-17 levels and PGR between gastric cancer group and proximal gastric cancer group (P> 0.05). Conclusions Serum PGⅠ, G-17 levels and PGR can be used as screening serological markers for the diagnosis of gastric cancer in Uygur people in Xinjiang Uygur Autonomous Region. The changes of serum PGⅠ, G-17 levels and PGR are related with tumor stage and different pathological changes in Uygur patients with gastric cancer Relevant parts.