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目的：研究ｂｃｌ－２蛋白过度表达和染色体１８ｑ微卫星不稳定性（ＭＳＩ）在胃癌发生及发展过程中的作用。方法：采用ＰＣＲ为基础的方法和免疫组织化学技术检测５０例手术切除胃癌标本的微卫星不稳定性及ｂｃｌ－２蛋白表达。结果：胃癌ｂｃｌ－２蛋白表达阳性率为６０％（３０／５０）；ＭＳＩ检出率为３０％（１５／５０）；中～高分化腺癌ＭＳＩ发生率（５３．３％，８／１５）显著高于低分化腺癌（１６．２％，５／３１）（Ｐ＜０．０５）。ｂｃｌ－２蛋白表达与ＭＳＩ无显著相关。结论：ｂｃｌ－２蛋白过度表达及ＭＳＩ（１８ｑ）在胃癌的发生中均发挥重要作用，二者引起胃癌发生机制有所不同
Objective: To study the role of bcl-2 overexpression and chromosome 18q microsatellite instability (MSI) in the development and progression of gastric cancer. METHODS: Microsatellite instability and bcl-2 protein expression in 50 specimens of gastric cancer were detected by PCR and immunohistochemistry. Results: The positive rate of bcl-2 protein expression in gastric cancer was 60% (30/50); the detection rate of MSI was 30% (15/50); the incidence of MSI was moderate to well-differentiated adenocarcinoma (53.3%, 8/15). ) Significantly higher than poorly differentiated adenocarcinoma (16.2%, 5/31) (P < 0.05). There was no significant correlation between bcl-2 protein expression and MSI. CONCLUSIONS: Overexpression of bcl-2 protein and MSI (18q) play an important role in the development of gastric cancer, which may cause different mechanisms of gastric cancer.