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从石油污染土壤中筛选出2种优势细菌枯草芽孢杆菌(Bacillus subtillus)和多食鞘氨醇杆菌(Sphingobacterium multivolum),在实验室条件下7d后,2种菌液对原油降解率分别为69.9%和60.1%。将这2种微生物制成的混合菌液与固体草炭土以0.5:1的比例制备成固体微生物菌剂后,投放到辽河油田石油污染土壤中,进行了现场原油污染土壤的修复实验,并与活性污泥和自然降解相比较。结果表明,现场条件下修复2个月后,固体混合菌剂、活性污泥、自然降解实验对原油降解率分别为73.5%、41.4%和38.5%,固体菌剂的修复效果明显优于活性污泥和自然降解。
Two dominant bacteria, Bacillus subtillus and Sphingobacterium multivolum, were screened from petroleum contaminated soils. After 7 days of laboratory conditions, the crude oil degradation rates of the two kinds of bacteria were 69.9% And 60.1%. The mixed bacteria liquid and solid peat soil prepared by these two kinds of microorganisms were prepared into solid microbial inoculant at the ratio of 0.5: 1, then put into the petroleum polluted soil of Liaohe Oilfield, and the experiment of repairing the crude oil polluted soil was carried out. Activated sludge and natural degradation compared. The results showed that the degradation rate of crude oil was 73.5%, 41.4% and 38.5% respectively after being mixed for 2 months in the field. The results showed that the solid bacteria agent was more effective than the activated sludge Mud and natural degradation.