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粳稻品种“嘉花1号”经60Coγ射线辐照后,在其后代中筛选到一个黄叶的突变体(yl6),经过表型分析,发现该突变体幼苗期不论在低温(20℃)还是在高温(32℃)培养条件下,与野生型相比叶色均呈现出淡黄色,表明其为一温度不敏感突变体。光合色素含量测定结果显示,yl6突变体的黄叶突变性状主要是由叶绿素含量下降所导致。电镜结果显示,yl6突变体内叶绿素合成受阻且叶绿体的正常发育受到影响。遗传分析表明,该突变性状受一对隐性核基因(yl6)所控制。利用该突变体与籼稻“培矮64S”杂交产生的F2、F3群体中分离出的608个突变体型单株作为定位群体,结合SSR和CAPS分子标记将yl6基因定位在水稻第6染色体短臂上的CAPS1和RM2353分子标记之间,其物理距离约为271kb,目前该区域内没有发现与水稻叶绿素合成/叶绿体发育相关已知功能基因。本研究结果可为yl6基因的克隆和功能分析奠定了基础。
A yellow leaf mutant (yl6) was screened from japonica rice “Jiahua 1” after 60Coγ-ray irradiation. After phenotypic analysis, it was found that the mutant seedlings had no significant difference in low temperature (20 ℃ ) Or under high temperature (32 ° C) culture conditions, the leaf color showed a yellowish color compared to the wild type, indicating that it is a temperature-insensitive mutant. The result of photosynthetic pigment content showed that the yellow leaf mutation of the yl6 mutant was mainly caused by the decrease of chlorophyll content. Electron microscopy showed that chlorophyll synthesis was blocked in the yl6 mutant and the normal development of chloroplasts was affected. Genetic analysis showed that the mutant trait was controlled by a pair of recessive nuclear genes (yl6). 608 mutants isolated from the F2 and F3 populations derived from a cross between the mutant and Pei’ai 64S were used as the loci. SSR and CAPS markers were used to locate the yl6 gene in rice chromosome 6 The physical distance between the CAPS1 and RM2353 molecular markers on the arm is about 271 kb. At present, no known functional gene related to chlorophyll synthesis / chloroplast development was found in this region. The results of this study can lay a foundation for the cloning and functional analysis of yl6 gene.