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刺激Schaffer侧支,记录大鼠海马脑片CA_1区的突触前排放(presynaptic volley,PV)和突触后群锋电位(population spikes,PS),观察缺氧后PS和PV的变化及复氧30min后脑片PS的恢复,缺氧持续到PV消失1,2,3或4min,复氧后脑片恢复率分别为100％,11.5％,0％,0％。可见,缺氧后 PV 消失2min为损伤的关键时刻。提前1min终止缺氧,全部脑片的PS可以恢复,延迟1min终止缺氧,则全部脑片的PS不能恢复。这种方法是根据每个脑片的电反应确定其总的缺氧时间,故每个脑片的缺氧时间略有变动。与每次采用相同缺氧时间的传统方法相比,此方法脑片恢复率的稳定性与重复性均较好。用此方法发现美西律10和100μmol/L能增加复氧后PS恢复率,对突触功能的缺氧损伤具有保护作用。
Schaffer collaterals were stimulated to record presynaptic volley (PV) and population spikes (PS) in hippocampal CA1 area of rat hippocampal slices. Changes of PS and PV after hypoxia and reoxygenation Recovery of PS after 30min, hypoxia persisted until PV disappeared 1, 2, 3 or 4min. The recovery rates of recuperation were 100%, 11.5%, 0% and 0% respectively. Visible, disappearance of PV after hypoxia 2min for the critical moment of injury. 1min early termination of hypoxia, all slices of PS can be restored, delayed 1min termination of hypoxia, then all slices of PS can not be restored. This method is based on the electrical response of each slice to determine the total time of hypoxia, so the time of hypoxia in each slice varies slightly. Compared with the traditional method of using the same time of hypoxia each time, the stability and repeatability of this method are better. Using this method, we found that 10 and 100 μmol / L of mexiletine can increase PS recovery rate after reoxygenation and protect the synaptic function of hypoxic injury.