论文部分内容阅读
为探讨辐射增强细胞摄取卡铂的机制,作者对在富氧条件和低其条件下以1mM的卡铂液处理1小时后的V_(79)中国仓鼠肺纤维母细胞株细胞,分别单次照射(照射时细胞仍处卡拍液中)10～50Gy/6.45～32.46分钟的不同剂量,并对同样处置的对照组行假照射。照射后立即冲洗、取材、离心等一系列处置后用原子吸收光清法测定细胞内的总铂量。结果所有受照射的细胞的铂浓度都高于对照组,且细胞内铂浓度随照射剂量增大而增高,在细胞内铂浓度与照射剂量之间存在明显正相关(富氧时r=0.9990,低
To investigate the mechanism of radiation-induced cellular uptake of carboplatin, we performed a single irradiation of V_ (79) Chinese hamster lung fibroblast cell line treated with 1 mM carboplatin in oxygen-rich condition and hypodermic conditions (Irradiated cells are still card-making liquid) 10 ~ 50Gy / 6.45 ~ 32.46 minutes of different doses, and the same treatment of the control group false illumination. Rinse immediately after irradiation, drawing, centrifugation and a series of treatment with atomic absorption spectrophotometry to determine the total intracellular platinum. Results All the irradiated cells had higher concentrations of platinum than those of the control group. The concentration of intracellular platinum increased with the increase of irradiation dose. There was a significant positive correlation between the concentration of intracellular platinum and irradiation dose (r = 0.9990, low