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目的观察经颅磁刺激(TMS)对大鼠大脑中动脉栓塞后神经功能恢复和健侧突触结构参数变化的影响,探讨其代偿机理。方法24只雄性SD大鼠应用线栓法建立大鼠大脑中动脉栓塞(MCAO)模型,随机分为TMS组和对照组,前者给予TMS治疗28d,后者常规饲养,观察大鼠神经功能恢复情况和健侧大脑感觉运动皮质区突触超微结构参数的变化。结果TMS组大鼠治疗后神经功能恢复优于对照组(P<0.05),电镜下观察到TMS组大鼠脑感觉运动皮质区突触界面曲率、突触后致密物质(PSD)厚度增加,突触间隙变窄,与对照组比较,差异有统计学意义(P<0.05)。结论TMS可促进脑缺血大鼠神经功能的改善,其机制可能与健侧突触超微结构参数的改变有关。
Objective To observe the effects of transcranial magnetic stimulation (TMS) on neurological function recovery and contralateral synaptic structural parameters after middle cerebral artery occlusion in rats, and to explore its compensatory mechanism. Methods Twenty-four male Sprague-Dawley rats were randomly divided into two groups: TMS group and control group. The former was given TMS for 28 days, and the latter was fed on a regular basis to observe the recovery of neurological function And synaptic ultrastructure parameters of motor cortex in the contralateral brain. Results The neurological function recovery of TMS group was better than that of the control group (P <0.05). The synaptic interface curvature and the thickness of the postsynaptic density substance (PSD) in the sensory motor cortex of TMS group were observed under electron microscope. Tactile space narrowing, compared with the control group, the difference was statistically significant (P <0.05). Conclusion TMS can promote the improvement of neurological function in rats with cerebral ischemia, the mechanism may be related to the changes of contralateral synaptic ultrastructure parameters.