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目的 探讨ｂｃｌ２ ，ｐ５３ 基因和细胞凋亡在胰腺癌发病机制中的作用以及它们之间相互关系．方法 应用ＡＢＣ 免疫组化技术检测５０ 例胰腺癌中Ｂｃｌ２ 和Ｐ５３ 蛋白表达，运用原位末端标记法观察肿瘤中细胞凋亡数量．结果 Ｐ５３ 蛋白表达阳性率为５４ ％ ，临床Ⅰ期阳性率（２６－７ ％ ）却显著低于Ⅱ期（６１－１ ％ ） 和Ⅲ＋ Ⅳ期（７０－６ ％ ，Ｐ＜ ０－０５） ；Ｂｃｌ２蛋白表达阳性率为６４ ％ ，临床Ⅰ期阳性率（９３－３ ％ ） ，显著高于Ⅱ期（５５－６ ％ ） 和Ⅲ＋ Ⅳ期（４７－１ ％ ，Ｐ＜ ０－０５） ；组织学Ⅲ级癌细胞中凋亡指数明显高于Ⅰ，Ⅱ级（ Ｐ＜ ０－０５） ，Ｂｃｌ２ 蛋白阴性病例中凋亡指数明显高于Ｂｃｌ２ 阳性者（ Ｐ＜ ０－０１） ．结论 Ｂｃｌ２ 是通过抑制细胞凋亡参与肿瘤的生长过程，Ｂｃｌ２和Ｐ５３ 蛋白表达之间 存在密切负相关（τ＝ － ０－１７４７ ，Ｐ＜ ０－０５） ．
Objective To investigate the roles of bcl2, p53 genes and apoptosis in the pathogenesis of pancreatic cancer and their interrelationships. Methods ABC immunohistochemical technique was used to detect the expression of Bcl2 and P53 protein in 50 cases of pancreatic cancer. The in situ end labeling method was used to observe the amount of apoptosis in the tumor. Results The positive rate of P53 protein expression was 54%. The positive rate of clinical phase I (26-7 %) was significantly lower than that of phase II (61-1%) and III + IV phase (70-6 %, P <0-05). The positive rate of Bcl 2 protein expression was 64%, and the positive rate in clinical stage I (93-3%) was significantly higher than that in stage II (55-6 %) and stage III+ IV (47-1 %, P<0-05). The apoptotic index in grade III cancer cells was significantly higher than that in grades I and II (P < 0-05), and the apoptosis index was significantly higher in patients with negative Bcl 2 protein than in those with positive Bcl 2 (P <0-01). ). Conclusion Bcl2 is involved in tumor growth by inhibiting apoptosis. There is a close negative correlation between Bcl2 and P53 protein expression (τ= -0-1747, P< 0-05).