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用扫描电镜观察松针褐斑病菌(Lecanosticta acicola)分生孢子在针叶表面萌发和侵入途径及时间,结果表明分生孢子在马尾松、湿地松和黑松针叶表面均能萌发,芽管通过针叶表面的气孔侵入针叶,侵入盛期是接种后3～4天。抗病的马尾松在感病后的针叶中过氧化物酶活性能迅速提高,而感病的湿地松和黑松针叶中过氧化物酶活性没有明显变化。马尾松、黑松和湿地松针叶中过氧化物酶活性水平与它们本身的抗病性无关,湿地松不同单株的针叶中过氧化物酶活性水平也不能反映它们相应的感病性。
Scanning electron microscope (SEM) was used to observe the germination and invasion of conidia of Lecanosticta acicola on the coniferous surface. The results showed that conidia could germinate on the surface of Pinus massoniana, Pinus elliottii and Pinus thunbergii, Stomatal invasion on the leaf surface of needles, invasive peak is 3 to 4 days after inoculation. The resistance of P. massoniana to the susceptible needle peroxidase activity can be rapidly increased, while susceptible Pinus elliottii and Pinus thunbergii peroxidase activity did not change significantly. The levels of peroxidase in Pinus massoniana, Pinus thunbergii and Pinus elliottii had no correlation with their own disease resistance. The levels of peroxidase in the needles of Pinus elliottii could not reflect the corresponding susceptibility.