论文部分内容阅读
目的：研究维生素Ａ增强人体免疫功能的机理。方法：采用ＲＴ－ＰＣＲ技术，检测分析人视黄酸受体（ｈＲＡＲｓ）基因在脐血和成人外周血淋巴细胞（ＰＢＬ）的表达，及丝裂原和视黄酸（ＲＡ）对其的调节。结果：人外周血淋巴细胞主要表达ｈＲＡＲα亚型基因，ｈＲＡＲγ亚型基因也有低水平转录；ＰＨＡ刺激２４小时脐血淋巴细胞（ＣＢＬ）ｈＲＡＲα和ｈＲＡＲγｍＲＮＡ都较刺激前显著升高（Ｐ＜０．０５）；在ＰＨＡ或ＳＡＣ刺激同时加入生理浓度ＲＡ，ＣＢＬｈＲＡＲαｍＲＮＡ较单用ＰＨＡ（Ｐ＜０．０１）或ＳＡＣ刺激明显增高（Ｐ＜０．０５）；ＰＢＬ的ｈＲＡＲｓ基因表达受丝裂原和ＲＡ的调节变化不大。结论：维生素Ａ促进免疫功能的机制中，调节免疫细胞中ＲＡＲｓ的表达可能是一条重要途径
Objective: To study the mechanism of vitamin A in enhancing human immune function. Methods: The expression of human retinoic acid receptor (hRARs) gene in cord blood and adult peripheral blood lymphocytes (PBL) was detected by RT-PCR, and its regulation by mitogen and retinoic acid (RA) . Results: Human peripheral blood lymphocytes mainly expressed hRARα subtype and hRARγ subtype was also transcribed at low level. The hRARα and hRARγ mRNA levels of cord blood lymphocytes (CBL) were significantly increased at 24 h after PHA stimulation (P <0.05) (P <0.01) or SAC stimulation (P <0.05). The expression of hRARs gene in PBL was affected by mitogen and RA Little change in regulation. Conclusion: In the mechanism of vitamin A promoting immune function, regulating the expression of RARs in immune cells may be an important way