为开发西红花转录组SSR标记,利用MISA软件筛选了西红花球茎转录组测序获得的29 572条Unigenes,对其SSR信息进行分析;利用Primer 3.0软件设计SSR引物,并随机选取30对SSR引物对6个西红花品种进行SSR引物筛选及多态性分析。结果发现,在西红花转录组中共搜索到个7 804个SSR位点,分布于6 306条Unigenes,SSR发生频率为18.73%,平均每5.85 kb含有1个SSR;SSR重复基元中单核苷酸重复出现频率最高,占总SSR的47.83%,其次为三核苷酸(32.94%)和二核苷酸重复(17.41%);二核苷酸重复基元以AG/CT为主(86.68%),三核苷酸重复基元以AAG/CTT为主(24.43%)。利用Primer 3.0软件共设计出11 508对候选引物,随机选择30对引物对6个西红花品种进行SSR引物筛选及多态性分析。30对引物均能扩增出预期大小的条带,有效扩增效率为100%,30对引物在6份西红花品种间未表现出多态性。本研究开发的SSR标记可为西红花遗传图谱构建、抗病虫及抗逆功能基因定位、分子标记辅助育种及西红花遗传多样性分析等提供丰富的候选标记。 In order to develop the SSR marker of saffron transcriptome, 29 572 Unigenes were screened by MISA software for SSR analysis. Primer 3.0 software was used to design SSR primers and randomly selected 30 pairs of SSR Primers for SSR primer screening and polymorphism analysis of 6 crocus sativus cultivars. A total of 7 804 SSR loci were found in 6 306 Unigenes. The frequency of SSR was 18.73% and the average number of SSRs was 5.85 kb. SSR repeats included mononuclear The repeat frequency of nucleotide was the highest, accounting for 47.83% of the total SSR, followed by trinucleotide (32.94%) and dinucleotide repeat (17.41%). The dinucleotide repeat motifs were mainly AG / CT %), Trinucleotide repeat motif to AAG / CTT (24.43%). A total of 11 508 pairs of primers were designed by Primer 3.0 software. 30 primer pairs were randomly selected for SSR primer screening and polymorphism analysis of 6 crocus sativus cultivars. 30 pairs of primers could amplify the expected size of the band, the effective amplification efficiency was 100%, 30 pairs of primers in 6 varieties of crocus did not show polymorphism. The SSR markers developed in this study can provide rich candidate markers for the construction of the genetic map of saffron flower, resistance gene locus of pest and disease resistance, molecular marker-assisted breeding and analysis of genetic diversity of saffron.