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目的:构建人肝再生增强因子()真核表达质粒,免疫家兔,获得抗多抗血清。hALRhALR方法:将已构建的人肝再生增强因子()重组质粒,经扩增,亚克隆入真核表达质粒,构建重hALR PET11-hALRPCR pcDNA3.1 pcDNA3.1-hALR组质粒。中量制备重组质粒及对照质粒,以只免疫家兔,取血并分离血清,进行蛋白质pcDNA3.1-hALR pcDNA3.11.08mg/印迹分析。Western blot结果:构建了真核表达质粒,此重组质粒诱发了家兔抗抗体的产生。pcDNA3.1-hALRhALR结论:说明构建的重组质粒在体内有表达能力,为进一步研究的生物学活性及某些疾病的治疗奠定了基础。 hALR
OBJECTIVE: To construct eukaryotic expression plasmid of human augmenter of liver regeneration and to immunize rabbits to obtain anti-polyclonal antiserum. hALRhALR method: The constructed recombinant plasmid of human liver regeneration enhancement factor () was amplified and subcloned into the eukaryotic expression plasmid to construct the recombinant hALR PET11-hALRPCR pcDNA3.1-hALR plasmid. The recombinant plasmids and control plasmids were prepared in medium volume to immunize rabbits only, blood was drawn and serum was separated for protein pcDNA3.1-hALR pcDNA3.11.08mg / blot analysis. Western blot results: eukaryotic expression plasmid was constructed, and the recombinant plasmid induced rabbit anti-antibody production. pcDNA3.1-hALRhALR Conclusion: The constructed recombinant plasmids have the ability to express in vivo, which lays the foundation for the further study of the biological activity and the treatment of certain diseases. hALR