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应用反相高效液相色谱法测定人血清中假尿苷（ＰＤ）的含量，色谱柱为Ｎｏｖａ－ＰａｋＣ１８３．９ｍｍ×１５０ｍｍ，流动相为０．０４ｍｏｌ／Ｌ磷酸二氢钾缓冲液（ｐＨ４．０），检测波长为２６３ｎｍ，线性范围为０．７～６．８μｍｏｌ／Ｌ，回收率为９３．５０％，日间误差ＣＶ＝３．１１％（ｎ＝６）。同时测定了部队体检正常人血清中ＰＤ的浓度，并用于临床观察肝炎、肾病、肺癌等多种疾病以及Ｈｅ－Ｎｅ激光治疗前后患者血中ＰＤ含量的变化。正常人血中ＰＤ的浓度无性别差异，成年人的正常值与文献一致。肾脏疾病中以尿毒症患者血清中的假尿苷升高最为明显，３例肺癌患者也都有升高的趋势。而我院开展的Ｈｅ－Ｎｅ激光治疗前后无明显的变异，证明激光对细胞修饰没有显著的影响。
The content of pseudouridine (PD) in human serum was determined by reversed-phase high performance liquid chromatography. The column was Nova-Pak C18 3.9 × 150 mm, the mobile phase was 0.04 mol / L potassium dihydrogen phosphate buffer (pH 4.0 ), The detection wavelength was 263nm, the linear range was 0.7 ~ 6.8μmol / L, the recovery was 93.50%, and the daytime error CV was 3.11% (n = 6). At the same time, the concentration of PD in the blood of normal military unit was measured and used to observe the changes of PD in patients with hepatitis, nephropathy, lung cancer and other diseases before and after He-Ne laser treatment. The concentration of PD in normal human blood has no gender difference, and the normal value of adults is consistent with the literature. In patients with uremia uremia in patients with elevated serum uridine in the most obvious, three cases of lung cancer patients also have an increasing trend. The He-Ne laser treatment in our hospital before and after no significant changes, that laser modification of cells had no significant effect.