论文部分内容阅读
目的 :探讨卡托普利与基质金属蛋白酶 9(MMP 9)在重症急性胰腺炎 (SAP)微循环障碍的相关机制并证实卡托普利在SAP微循环障碍的价值。方法 :SD大鼠随机分为 :假手术组 (n =1 0 )和SAP组 (n =1 0 ) ,及MMP抑制剂干预组 (n =1 0 )。检测各组大鼠胰损伤评分、胰湿重、血清淀粉酶量、腹腔灌洗液细胞数、腹腔灌洗液中和血液中Evens染料量比、胰组织MMP 9表达等有关指标。结果 :SAP组大鼠胰损伤评分、胰湿重、血清淀粉酶量、腹腔灌洗液细胞数、腹腔灌洗液中和血液中Evens染料量比均明显高于假手术组和MMP组 (P <0 .0 1 )。胰腺组织内MMP 9的阳性表达率 ,SAP组、假手术组、MMP组三组分别为 1 0 0 %、1 1 %和 2 5 % ,组内比较差异有显著性意义 (P <0 .0 1 )。结论 :MMP 9在SAP中因炎性细胞受炎症因子的激活而大量释放 ,与SAP时微循环障碍有密切关系。应用卡托普利可减轻该病理过程 ,从而成为SAP治疗的新靶点
Objective: To investigate the relationship between captopril and matrix metalloproteinase 9 (MMP 9) in microcirculation disturbance of severe acute pancreatitis (SAP) and to confirm the value of captopril in SAP microcirculation disturbance. Methods: SD rats were randomly divided into sham operation group (n = 10) and SAP group (n = 10), and MMP inhibitor intervention group (n = 10). The pancreatic injury score, the wet weight of pancreas, the amount of amylase in serum, the number of peritoneal lavage fluid, the amount of Evens dye in peritoneal lavage fluid and blood, the expression of MMP 9 in pancreatic tissue were detected. Results: The pancreatic injury score, the wet weight of pancreas, the amount of amylase in serum, the number of peritoneal lavage fluid, the amount of Evens dye in peritoneal lavage fluid and blood in SAP group were significantly higher than those in sham operation group and MMP group <0 .0 1). The positive expression rate of MMP 9 in pancreatic tissue was 100%, 11% and 25% in SAP group, sham operation group and MMP group respectively, there was significant difference between the two groups (P <0. 0 1 ). Conclusion: The release of MMP-9 in SAP by inflammatory cytokines is mainly due to the activation of inflammatory cytokines, which is closely related to the microcirculation disturbance in SAP. The use of captopril can reduce the pathological process, which became a new target for SAP treatment