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[目的 ] 研究海南地区不同人群TTV感染情况。 [方法 ] 对 162例城市一般人群、15 3例农村少数民族、12 0例献血员、96例丙氨酸氨基转移酶异常者、68例血透析者、92例非甲～庚型肝炎病人、10 2例甲～庚型肝炎病人、181例静脉毒瘾者 ,采用巢式PCR法检测血清中TTVDNA ,并对两株TTV的部分基因序列进行了测定。 [结果 ] 海南地区以上 8种人群中的阳性率依次为 10 5 %、8 5 %、9 2 %、11 5 %、17 6%、40 2 %、2 6 5 %、3 8 7%,各类人员之间差异非常显著 (P <0 0 1) ;TTV阳性者甲～庚型肝炎病毒合并感染率平均达 5 1 5 2 %,以HBV、HCV为常见 ,在2 7例合并感染中 ,急、慢、重型肝炎分别为 3 3 3 %、2 5 6%、40 7%,2 6例单纯TTV感染者无明显的肝炎表现。两株TTVDNA序列与日本株相应位置核苷酸序列的同源性大于 98%。 [结论 ] 海南地区不同人群TTV感染均常见 ,非甲～庚型肝炎、静脉毒瘾者是高危人群。
[Objective] To investigate the prevalence of TTV infection in different populations in Hainan. [Method] A total of 162 cases of urban general population, 15 3 rural ethnic minorities, 120 blood donors, 96 cases of abnormal alanine aminotransferase, 68 hemodialysis patients, 92 non-A ~ G hepatitis patients, One hundred and two cases of A ~ G hepatitis and 181 cases of intravenous drug addicts were detected by nested PCR. The serum TTTVNA was detected and the partial sequences of two TTV genes were determined. [Results] The positive rates among the 8 populations above Hainan were 105%, 85%, 92%, 115%, 176%, 402%, 265%, 388%, respectively (P <0.01). The average infection rate of A ~ G hepatitis virus in TTV-positive patients was 51.52%, HBV and HCV were the most common, and in 27 cases of co-infection, The acute, chronic and severe hepatitis were 33.3%, 25.6% and 40.7% respectively. There were no obvious hepatitis manifestations in 26 TTV patients. The homologies of two TTVDNA sequences with the corresponding nucleotide sequences of Japanese strains were more than 98%. [Conclusion] TTV infection in different populations in Hainan are common. Non-A-Hepatitis G and intravenous addicts are at high risk.