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目的探讨小补心汤总黄酮(XBXT-2)含药血清的神经保护作用。方法采用血清药理学方法分别制备大鼠的空白血清、氟西汀(10 mg/kg)和XBXT-2(25,50,100,200 mg/kg)含药血清。采用皮质酮损伤大鼠肾上腺嗜铬细胞瘤细胞(PC12)、人神经母细胞瘤细胞(SH-SY5Y)及原代培养大鼠海马神经元为体外药理学模型,应用ATP生物发光法检测XBXT-2及氟西汀含药血清对损伤细胞存活率的影响。结果皮质酮(50～300μmol/L)能够浓度依赖性地抑制SH-SY5Y的存活,氟西汀与XBXT-2(25,50 mg/kg)含药血清能显著提高皮质酮(100μmol/L)损伤的SH-SY5Y的存活率(P<0.01,P<0.05,P<0.01);与空白对照组比较,200μmol/L皮质酮导致PC12的存活率显著下降(P<0.01),氟西汀与XBXT-2(25,50,100 mg/kg)含药血清能显著对抗这种损伤(P<0.05,P<0.01,P<0.05);100μmol/L皮质酮使原代海马神经元存活率显著下降(P<0.001),XBXT-2(25、50 mg/kg)对这种损伤具有显著的保护作用(P<0.05,P<0.001),其作用与阳性药氟西汀含药血清相当。结论 XBXT-2含药血清对皮质酮所致的神经细胞损伤具有明显保护作用,这可能是其抗抑郁效应的重要细胞机制之一。
Objective To explore the neuroprotective effect of XBXT-2-containing serum. Methods Serum pharmacology methods were used to prepare blank serum, fluoxetine (10 mg / kg) and XBXT-2 (25, 50, 100, 200 mg / kg) Adrenal pheochromocytoma cells (PC12), human neuroblastoma cells (SH-SY5Y) and primary cultured rat hippocampal neurons were induced by corticosterone in vitro pharmacology model. ATP bioluminescence was used to detect the expression of XBXT- 2 and fluoxetine-containing serum on injured cell survival rate. Results Corticosterone (50 ~ 300μmol / L) could inhibit the survival of SH-SY5Y in a concentration-dependent manner. Fluoxetine and XBXT-2 (25,50 mg / (P <0.01, P <0.05, P <0.01). Compared with the blank control group, the survival rate of PC12 induced by 200 μmol / L corticosterone was significantly decreased (P <0.01) The drug-containing serum of XBXT-2 (25, 50 and 100 mg / kg) could significantly resist this injury (P <0.05, P <0.01, P <0.05); the survival rate of primary hippocampal neurons was significantly decreased P <0.001). XBXT-2 (25 and 50 mg / kg) had a significant protective effect on this injury (P <0.05, P <0.001), which was comparable to the positive drug-containing fluoxetine-containing serum. Conclusion XBXT-2-containing serum has a significant protective effect on cortisone-induced neuronal injury, which may be one of the important cellular mechanisms of its antidepressant effect.