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目的:研究胆囊良恶性上皮病变中增殖细胞核抗原(PCNA)、周期蛋白D1(CYCLIND1)的表达情况,探讨胆囊癌的发生与胆囊腺瘤及胆囊结石的关系.方法:应用免疫组织化学SP法,对64例胆囊上皮病变进行检测.结果:在胆囊炎上皮中,PCNA、CYCLIND1的表达与腺瘤大小、结石存在与否、结石的大小有关.结论:较大的胆囊腺瘤(直径≥1.0CM)及合并结石的腺瘤具有较强的癌变倾向;胆囊结石对胆囊粘膜的长期刺激具有潜在的致癌倾向.“,”Objective:Our paper was designed to study the expression of proliferative cellular nuclear antigen (PCNA), Cyclin D1 in benign and malignant lesion of the gallbladder, and to study the relationship between the carcinogenesis of the gallbladder and the benign epithelial lesion associated with gall stone.Methods:The expressions of PCNA and Cyclin D1 in 55 cases of benign lesion and 9 cases of malignant lesion of gallbladder epithelium were studied by immunohistochemical method (S-P method). Results: Between the cholecystocarcinoma group and the cholecystitis group, there was a prominent difference in the average positive index of PCNA and the positive rate of Cyclin D1, but the difference was not significant between the cholecystocarcinoma group and the cholecystoadenoma group. In cholecystoadenoma, the expression of PCNA and Cyclin D1 had the relationship with the size of the adenoma and the existence of the gall stone. Then in cholecystitis, the expression was associated with both the existence and the size of the gall stone.Conclusion: The bigger cholecystoadenoma (d≥1.0 cm) and the cholecystoadenoma associated with gall stone might have intensive carcinogenesis tendency. The long term stimulation of gall stone to the gall mucosa also played as the potential carcinogenesis role.