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本文对于改变胰蛋白酶专一性的方法进行了研究,通过对胰蛋白酶静电性质的计算找到了一种改变表面碱性氨基酸来达到使酶选择性地与精氨酸底物反应的途径,并给出了推荐的突变体表列。
In this paper, the method of changing the specificity of trypsin was studied. By calculating the electrostatic properties of trypsin, we found a way to change the surface basic amino acids to allow the enzyme to selectively react with arginine substrates, The recommended mutant list.