芦荟遗传转化体系的建立及人胰岛素样生长因子I转化芦荟的初步研究

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本项目将人工半合成的人类胰岛素基因转化芦荟,并研究该基因在植物体内表达;试图获得有人类胰岛素表达、对糖尿病等疾病具有食疗作用的芦荟新品种,为人类疾病的防治提供一条经济有效的新途径;为以芦荟为植物反应器,生产人类胰岛素奠定基础;使芦荟这一热带药用植物资源能够更好地服务于人类。 植物表达载体的构建。选用单子叶植物启动子Ubiquitin和空白载体PUC1301,PVKH-35S-GUS-PA构建了植物表达载体PUC1301-U和PVKH-U。选用植物偏爱密码子改造IGF基因启始
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