用SSR标记鉴定大赖草染色体及其片段

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利用定位于小麦七个部分同源群上的337对SSR引物对大赖草、普通小麦基因组DNA进行扩增分析,结果表明有113对引物可检测到两个物种间的多态性。利用上述多态性引物进一步对小麦—大赖草Lr.2、Lr.7、Lr.14异附加系进行分析表明,小麦7BL上的SSR引物gwm112可用来追踪大赖草染色体Lr.2,并揭示Lr.2与小麦第七部分同源群有同源性;5A上的SSR引物gwm205,gwm156、5D上的SSR引物gwm212在Lr.7和Lr.14中均有相同的扩增位点,揭示Lr.7和Lr.14与小麦第五部分
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