论文部分内容阅读
The fish immune system structure and function are not well studied like mammalian models and recent researches showed that a lot of factor not common between freshwater and other fish and there are many disease and microbes affect some fish and cause a sever effect where other fish resist it. Our work focused on Nile tilapia as a model for freshwater bony fishes, where Nile tilapia consider one of the major massive culture fish all over the world and little is known about is immune system from the structure and functional point of view, our work focused on identify the tilapia immune component as PRRs and fibroblast growth factor receptor families and check either its presence of absence and if present check it to detect either it consider functional genes or nonfunctional genes, then make analysis to its protein structures and transmembrane structure and trying to identifying the sites of mutation between Nile tilapia and other available fish models, so we can get more clear data about fish evolution, then trying to study the effect of this mutant sites on immune response. We detect, identified, cloned and expressed the complete sequence of the Nile tilapia toll like receptor type1,2,3,5which reported as tilapia TLR-1/TLR-2/TLR-3/TLR-5sequences in various Nile tilapia tissues, We also success to detect and clone Nile tilapia fibroblast growth factor receptor-1, Nile tilapia fibroblast growth factor receptor-2and Nile tilapia fibroblast growth factor receptor-3. The phylogenic analysis showed that our cloned genes are orthologs to the known TLRs and FGFRs. We reported a degree of genetic variation in TLRs genes among fish and vertebrates models we also found patterns of positive selection acting on specific amino acid sites that could be linked to species-specific differences in pathogen-associated molecular pattern recognition. This study provides evidence for the evolutionary patterns and implications of TLR1;2;3;5and FGFR1,2,3members polymorphism in new fish model specie and extends the list of available fish immunogenetic genes.