【摘 要】
：
[目的]探讨p14ARF基因甲基化水平与胶质瘤发生和预后的关系及其与P53蛋白表达的相关性。 [方法]采用甲基化特异性PCR法（Methylation specific polymerase chain reactionMSP）检测33例胶质瘤和12例正常脑组织中p14ARF基因启动子区甲基化水平。采用免疫组织化学Envision二步法检测58例胶质瘤和12例正常脑组织中p14ARF和P53蛋白
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[目的]探讨p14ARF基因甲基化水平与胶质瘤发生和预后的关系及其与P53蛋白表达的相关性。
[方法]采用甲基化特异性PCR法（Methylation specific polymerase chain reactionMSP）检测33例胶质瘤和12例正常脑组织中p14ARF基因启动子区甲基化水平。采用免疫组织化学Envision二步法检测58例胶质瘤和12例正常脑组织中p14ARF和P53蛋白表达情况。
[结果]胶质瘤与正常脑组织中p14ARF基因启动子区甲基化率分别为39.4％（13/33）和0（0/12），两组间差异具有统计学意义（P<0.01）。低级别组胶质瘤中p14ARF基因启动子区甲基化率（6/15）与高级别组（7/18）无明显差异（x2=0.004，P>0.05）。p14ARF基因启动子区甲基化情况与患者预后无明显相关性（x2=0.004，P>0.05）。P53蛋白表达在胶质瘤和正常脑组织中的阳性率分别为56.9％（33/58）和8.3％（1/12），两组间差异具有统计学意义（x2=7.544，P<0.01），且胶质瘤高级别组中表达明显高于低级别组（x2=5.115，P<0.05）。胶质瘤P53蛋白表达与p14ARF基因启动子区甲基化呈负相关（r=-0.385，P<0.05）。
[结论]p14ARF基因启动子区甲基化与胶质瘤发生密切相关，可作为胶质瘤诊断的分子标志物之一。
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