The biocompatibility, durability, and strength, among others are some properties thatrender silk fibers of several uses such as biomedical (like sutures), pharmaceutical andprotective applications.Hence, for such applications silk may be reproduced into film,gel powder, and most recently, nanofibers.Bombyx mori is the most common specieof silkworms, yielding fibers of triangular-shaped cross-section with diameter 10-20μm.The regeneration process is aimed at giving fibroin with similar structure but withbetter properties relevant to intended applications.Among others, the degradation offibroin, and loss of secondary structural conformation during regeneration was ahypothesized shortcoming.The first objective of this research was to successfullydissolve B.mori silk fibers and then reclaim it into gel and film forms.The secondtask was to dissolve the regenerated films and consequently electrospin intonanofibers.Structural studies on the morphology and the secondary crystallinity werethe final rationale for the study;, to assess the structural conformational changes fromthe original fibers.Two different solvents were used as a control measure to studyconsistency.The silk fibers were dissolved and regenerated successfully usingaqueous LiBr and a ternary system of CaCl2:Water:Methanol.Electrospinning wasdone in formic acid, and the morphology of the resulting fibers was assessed byscanning electron microscopy (SEM).The crystalline secondary structure ofregenerated Bombyx mori fibroin (films and nanofibers) was evaluated using Fouriertransform infrared spectroscopy (FTIR) and Wide angle x-ray diffraction (WAXD).Results showed that untreated regenerated fibroin in film and nanofibers is generallyamorphous.It was also established that the regeneration process has a remarkableeffect on the molecular weight and crystallinity of fibroin.