灵芝GLUGT1基因的全长cDNA克隆和生物信息学分析

来源 :中药与天然药高峰论坛暨第十二届全国中药和天然药物学术研讨会 | 被引量 : 0次 | 上传用户:billdyj
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  本研究通过对灵芝的基因组测序结果进行分析,采用RT-PCR方法,对其中一条可能参与灵芝三萜合成的UDP-糖基转移酶基因转录本(GL25279)进行全长克隆,并对该基因编码蛋白的理化性质、蛋白二级结构及三维结构等进行了预测与分析.通过全长克隆,得到一条开放阅读框为l491 bp的eDNA序列,命名为GLUGT1.序列分析表明,该基因编码496个氨基酸,蛋白分子量为54.5544 kD,等电点为6.24,属于稳定蛋白.二级结构中α-螺旋占36.69%、β-折叠占11.49%、无规卷曲占51.81%.该蛋白不具有信号肽和跨膜区,最有可能定位在线粒体或者细胞质中.序列比对表明,该蛋白和云芝中的UDP-糖基转移酶EIW63452的相似度较高,为50%.本研究成功克隆并分析了灵芝UGT1的全长序列,为进一步研究灵芝三萜代谢途径奠定基础.
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