Objective The purpose of this study is to identify critical genes associated with septic multiple-trauma through comparingperipheral whole blood samples from multiple trauma patientswith and without sepsis.MethodsMicroarray data set was downloaded from Gene Expression Omnibus(GEO)database which includes 70samples,36from multiple trauma patients with sepsis and 34from multiple traumapatients without sepsis(as control).The data were pre-processed and differentially expressed genes(DEGs)were then screened out using packages of R.Functional analysis was performed for the DEGs with DAVID.Then interaction networks were established for the most up-and down-regulated genes using softwareHitPredict.Pathway enrichment analysis was carried out subsequently for the genes in the networks bysoftware WebGestalt.ResultsA total of 58DEGswere identified.The expression levels of PLAU(down-regulated)and MMP8(up-regulated)presentedthe largestfold changesand interaction networkswere established for them.Further analysis reveals that PLAT(plasminogen activator,tissue)and SERPINF2(serpin peptidase inhibitor,clade F,member 2),which interact with PLAU,play important roles in pathway of component and coagulation cascade.ConclusionWe speculate that PLAU is a major regulator in component and coagulation cascade and the down-regulation ofPLAUresults in dysfunction of the pathway and thus causes the incidence of sepsis.