Ginsenoside, a triterpenoid saponin, is considered to be the major active component with many beneficial bioactive effects on human health, such as anti-tumor, anti-stress, anti-aging, and also improves immune function.Due to its huge pharmaceutical properties, special attention has been paid to genes involved in ginsenoside biosynthesis for large scale production.Genome sequence has not been available for P.ginseng till now.The alternative method for functional genomic analysis is expressed sequence tags (EST).Recently, next-generation sequencing (NGS) technologies render large-scale EST analyses more feasible for its dramatically reduced cost and experimental complexity of sequencing.