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In looking to improve the quality of liquid chromatographic separations, a user easily becomes buried under acronyms and trade names for systems like UPLCTM, X-LCTM, RSLC, Acquity, and Infinity etc.In addition in the past years a dispute seems to emerge between usage of sub-2-micrometer totally porous particles and so-called, 2-3 μm porous shell particles in columns that deliver ultra-high separation efficiency.This situation does not help one to make the best choice and blur the view on optimal conditions for ones particular LC separation and analysis problem at hand.Fortunately nowadays, the acronym UHPLC, which stands for Ultra High Performance Liquid Chromatography, has become a common way to differentiate the newer LC separation techniques from HPLC which is limited in performance by the lower maximum operating pressure of such systems (400 bar compared with 1200 bar in UHPLC systems).