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It is widely accepted that anoikis resistance is the prerequisite of cancer cells metastasis.Elucidation of the mechanism of anoikis resistance remains a significant challenge.Elevated HBV DNA level is considered a strong risk predictor of HCC metastasis or recurrence.We had found that HBpreS2 (preS2) transfection facilities trail induced apoptosis, whether or not preS2 transfection could also facilities hepatoma cells to anoikis was verified in our lab.