C.azorensis H53214氢酶基因在产酸克雷伯氏菌HP1中的表达及对其产氢的影响

来源 :全国农业生物化学与分子生物学第十六届学术研讨会 | 被引量 : 0次 | 上传用户:vay_b
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  氢是一种有潜力的新型能源,微生物发酵制氢是清洁环保可再生的制氢方式。产酸克雷伯氏菌HP1是本研究室从70℃温泉中分离得到的一株高产氢活性菌株,该菌株属于肠杆菌科,其含有与大肠杆菌氢酶3相似的[NiFe]氢酶。该菌株主要由甲酸氢裂解复合酶体系(FHL)催化甲酸裂解,产生的氢及电子由FHL体系中的[NiFe]氢酶催化产生分子氢。[FeFe]氢酶可以利用糖酵解途径中所产生的NADH为底物产氢。因此将[FeFe]氢酶转入产酸克雷伯氏菌理论上可以大大提高该菌株的产氢效率。
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