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Assembling 1D nanostructure such as nanowires/nanorods/nanotubes into 2D macrostructure has generated much research interest due to its unique properties and wide applications.In this paper, flexible membranes were successfully fabricated using α-MnO2 nanowires synthesized through hydrothermal method.The effects of drying time and thickness on the mechanical properties of the membranes were studied and optimal preparing conditions were identified.Nano-indentation measurements show that the modulus of the as-prepared membrane reaches up to 12 GPa.In addition, thermal analysis shows that the membrane is thermally stable to withstand 500 ℃ or even higher annealing temperature.