Hox genes are well-known master regulators in segmental identity and organ specification during development.The expression and activity of Hox genes are critical to animal body plans in embryonic stage,but their roles in the post-embryonic development remain largely unexplored.Here,we took three wing-defective mutants,Wes,EM and Nc,all of which were caused by antp mutation,as material to explore how Antp to be involved in wing development in Bombyx mori.Firstly,the expression patterns of Antp was analyzed during wing disc development in Bombyx mori.We showed that BmAntp was expressed in both fore and hind wings with a similar expression profile.The gene went through a modest expression in fifth instar and then increased gradually after pupation and reached the peak before moth.The Q-PCR indicated that the expression of Antp is higher in the mutants compared with that in the wide-type.EMSA showed that in vitro Antp can bind to ap and vg gene as downstream targets,which indicate that BmAntp probably be involved in wing formation.Our research has offered a new insight of Hox gene putting in insect wing development,and the Bombyx wing might provide a useful system for understanding how Hox proteins select and regulate their target genes in organ specification.