@@ Immunosuppressive drug was used for most allo—liver recipients to prevent graft rejection.Tacrolimus(also known as FK506、is one of the most popular immunosuppressant for transplantation patients. As T lymphocyte specific targeted suppressant， tacrolimus could regulate the immune states of recipients and immune states of patients were hugely different from before transplantation operation The anti-nuclear antibody(ANA)is one of the most important autoantibody against nucleus or/and cytoplasm substance in the imbalance of the immune system，which Can occur in a number of dif.ferent autoimmune disorders including systemic lupus erythematosus and Sjogren’S syndrome，target specific protein antigens present in the body’s nucleated cells.Therefore.we selected liver transplantation patients with a long—term treatment of tacrolimus and control patients with matched liver diseases to detect serum ANA to evaluate the immunity state of allo-liver recipients.