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Immunoassays for heavy metals offer an accurate and alternative approach to traditional techniques for detection of Pb.In this study, a Pb-chelate was prepared with DTPA.The detection antigen (BTX-BSA) complex was linked to bovine serum album and the immunizing antigen (BTX-IgG) complex was linked to keyhole limpet hemocyanin (KLH).BALB/c mice were immunized with KLH-DTPA-Pb and spleen ceils from BALB/C mice were fused with Sp2/0 cells.One cell line (3F7) produced monoclonal antibodies with preferential selectivity and sensitivity for BSA-DTPA-Pb.This work presents a rapid and sensitive method for detecting Pb at low concentration.In this method, lead antibody is conjugated with magnetic beads to separate Pb from the blood and the other antibody, associated with quantum dots, is used to detect fluorescence.The Pb in blood can be quantified using the relationship between the QDs fluorescence intensity and the Pb in blood frequency following magnetic separation.The numerous advantages of detecting Pb at low concentration using the presented method include ease of preparation, low cost, fast detection,and high sensitivity.