Proteins and nucleic acids are dynamically organized in cells to realize their physiological functions with spatial and temporal orderliness.This type of elegant supermolecular assembly has inspired researchers to create molecular/biomolecular structures with dynamic organization outside of the cells.In particular,DNA nanotechnology has proven to possess extraordinary flexibility and convenience for "bottom-up" construction of exquisite nanostructures with high controllability and precision,which holds great promise in a wide range of applications,e.g.,nanofabrication and molecular electronics,in-vivo and in-vitro sensing and drug delivery.In this talk,I will present several examples of using tetrahedral DNA nanostructures(TDNs)for dynamic organization of biomolecules in vitro.TDNs are three-dimensional(3D)DNA architecture with high mechanical rigidity and structural stability,which are suitable for organization of higher-ordered nanocomplexes and nanodevices.As one of the examples,we employed single-particle tracking to visualize the internalization of TDNs,and dissect the cell entry pathways of these virus-like nanoparticles.In the second example,I hope to use the way that we employed TDNs to dynamically organize the biosensing interface,and realize macroscopic applications on diagnostics.