Caveolin-1调控肝癌细胞ST6Gal-I表达与肿瘤粘附研究

来源 :第四届泛环渤海生物化学与分子生物学会2013年学术交流会 | 被引量 : 0次 | 上传用户：k5105320

【摘要】

：

目的:探讨Caveolin-1对肝癌细胞ST6Gal-I表达的影响及与肿瘤粘附行为的关系.材料和方法:利用Rea-time PCR和Westem-blot等方法检测Caveolin-1在小鼠肝癌细胞系(Hca-F,Hepa1-6

【作者】

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汪淑晶于生金樊建慧张嘉宁

【机构】

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大连医科大学生物化学与分子生物学教研室,糖生物学研究所,大连,116044

【出处】

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第四届泛环渤海生物化学与分子生物学会2013年学术交流会

【发表日期】

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2013年8期

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目的:探讨Caveolin-1对肝癌细胞ST6Gal-I表达的影响及与肿瘤粘附行为的关系.材料和方法:利用Rea-time PCR和Westem-blot等方法检测Caveolin-1在小鼠肝癌细胞系(Hca-F,Hepa1-6等)中的表达情况;利用RNAi方法下调Caveolin-1在Hca-F细胞中的表达;利用Rea-time PCR等方法分析Caveolin-1表达下调对Hca-F细胞ST6Gal-I及细胞表面α2,6连接唾液酸的影响;利用Western-blot方法分析Caveolin-1表达下调对FAK介导的粘附信号传导通路的影响;利用细胞粘附实验分析Caveolin-1表达下调对Hca-F细胞粘附能力的影响;在下调Caveolin-1表达的Hca-F细胞重新表达Caveolin-1和ST6Gal-I,然后观察细胞粘附的变化情况.

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Caveolin-1调控肝癌细胞ST6Gal-I表达与肿瘤粘附研究

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